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. 2018 May 25;293(27):10561–10573. doi: 10.1074/jbc.RA117.001491

Figure 1.

Figure 1.

Validation of C6orf106 knockdown and overexpression in HeLa cells. a and b, HeLa cells were transfected with siRNAs targeting C6orf106 (siC6orf106) or a nontargeting control (siNEG) and assayed by qRT-PCR (a) or Western blotting (b) for C6orf106 expression levels. c, validation of C6orf106-FLAG expression by Western blotting. d, cell viability in HeLa cells treated with the indicated siRNAs or cDNAs. Values are normalized against cells treated with transfection reagent only (mock). Error bars for all graphs indicate ±1 S.D. of a minimum of three independent experiments; asterisks show significant differences as assessed by one-way ANOVA with Bonferroni post-test (*, p < 0.05; **, p < 0.01).