Figure 3.

C6orf106 predominantly targets IRF-driven transcription. HeLa cells were transfected with siRNAs for 24 h, then transfected with ISRE- (a) or NF-κB-firefly luciferase (luc) (b) and Renilla luciferase vectors for 20 h, and then stimulated with poly(I:C) for 6 h. Alternatively, HeLa cells were transfected with GFP or C6orf106-FLAG and ISRE- (c) or NF-κB-firefly luciferase (d) and Renilla luciferase vectors for 20 h and then stimulated with poly(I:C) for 6 h. All cell lysates were assayed for luciferase activity, normalized to the transfection control Renilla luciferase. Error bars indicate ±1 S.D. of triplicate experiments; asterisks indicate significant differences as determined by Student's t test (*, p < 0.05; **, p < 0.01).