Figure 5.

C6orf106 does not alter cellular protein levels of IRF3 and NF-κB. HeLa cells were transfected with cDNAs for 20 h (a) or siRNAs for 40 h (b). Cells were then pretreated for 1 h with CHX (20 μg/ml) or MG132 (10 μm) and then stimulated with poly(I:C) for 6 h. Cells were lysed, and cellular levels of IRF3, p65, and C6orf106 (endogenous or FLAG-tagged) were measured by Western blotting. c, immunoblot showing expression levels of cyclin B1 and actin in HeLa cells treated for the indicated times with CHX (20 μg/ml) or MG132 (10 μm).