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C6orf106 reduces nuclear levels of the transactivator proteins p300 and CBP. Nuclear and cytosolic fractions were isolated from HeLa cells expressing C6orf106-FLAG (a) or knocked down with siRNAs targeting C6orf106 (b) and stimulated with poly(I:C) as shown. Equal amounts of nuclear lysates were probed for members of the enhanceosome complex as shown. c, cytosolic fractions were also probed for the nuclear transactivators CBP/p300.
