Figure 5.

Human embryonic stem cells expressing the XLID OGT-TPR variants show no gross changes in steady-state O-GlcNAc, OGT, and OGA levels. The endogenous WT or variant OGT expressing RUES-1 hES cell lysates was probed for O-GlcNAc using the anti-O-GlcNAc antibody, CTD 110.6 (a), or a second anti-O-GlcNAc antibody, RL2 (b). Samples were also probed for OGT and OGA using the antibodies DM-17 and G-12 respectively (c) or antibodies F-12 and 14711-1-AP, respectively (d). All membranes were probed using an anti-β-actin antibody as a loading control. Blots shown are representative of three biological replicates. IB, immunoblot.