Table 2.

Michaelis-Menten kinetics of the XLID-associated TPR variants of OGT

OGT variant	Km	kcat	kcat/Km
	μm	s−1	s−1 m−1
WT	7 ± 0.6	4.5 × 10−3 ± 0.1 × 10−3	642.86
L254F	13 ± 3	3.5 × 10−3 ± 0.3 × 10−3	269.23
A259T	9 ± 0.6	3.5 × 10−3 ± 0.07 × 10−3	388.89
R284P	9 ± 1	3.5 × 10−3 ± 0.1 × 10−3	388.89
A319T	14.5 ± 1.5	3.3 × 10−3 ± 0.1 × 10−3	227.59
E339G	12 ± 1.5	4.0 × 10−3 ± 0.2 × 10−3	333.33