Table 1.

Select MEC-10 mutations alter channel activity

Xenopus oocytes were injected with cRNA mixtures of MEC-4 A713T (MEC-4d), MEC-2, and MEC-6 in the presence of WT MEC-10 or individual MEC-10 mutants. Basal currents (before the initiation of LSS stimulation) were measured at −60 mV by perfusing oocytes with NaCl-110 TEV solution. At the end of each recording, 20 μm benzamil (50 μm for channels without MEC-10) was added to the bath to determine leak currents. n indicates the number of oocytes assessed for each group. Statistical comparisons of whole-cell Na⁺ currents measured in oocytes expressing WT MEC-10 versus mutant channels were determined with one-way ANOVA followed by a Bonferroni test (*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, not significant).

Mutation	INa+	S.D.	n	p
	μA	μA
WT MEC-10	−2.52	2.49	92
No MEC-10	−4.28	2.92	84	*
G676R	−0.72	0.30	14	****
L679R	−0.51	0.19	15	****
G680R	−0.59	0.18	15	****
G684E	−0.43	0.14	15	****
A673C	−4.08	2.81	15	NS
D674C	−1.08	1.14	10	**
F675C	−2.11	1.43	10	NS
G676C	−2.14	1.56	11	NS
G677C	−0.80	0.71	9	**
H678C	−0.60	0.33	13	****
L679C	−0.96	0.96	11	**
G680C	−0.52	0.15	10	***
L681C	−1.58	1.15	15	*
W682C	−1.61	0.74	15	*
S683C	−1.40	1.69	15	**
G684C	−0.30	0.16	15	****
V685C	−1.52	0.57	10	NS
S686C	−0.36	0.28	12	****
V687C	−1.86	1.04	10	NS