FIGURE 2.

CXCL17 is unable to promote G protein–mediated signaling via isoforms of human GPR35. Either the short, GPR35a (A and B), or the N-terminally extended, GPR35b (C), isoforms of human GPR35 were expressed in HEK293 cells along with the chimeric G protein α subunits G_q–G₁₃ (A) or G_q–G_i1/2 (B and C). Following incubation with the indicated concentrations of zaprinast (filled circles), lodoxamide (filled triangles), or CXCL17 (open symbols) for 30 min, levels of inositol phosphates were measured.