FIGURE 3.

GPR35 is readily expressed in L1.2 cells but does not mediate chemotactic responses to CXCL17. (A) represents typical staining profiles obtained for L1.2 cells transiently transfected with the 3xHA GPR35 plasmid. Staining with isotype control is shown as an open histogram, and anti-HA staining is shown as a filled histogram. (B) shows the migratory responses of GPR35 expressing L1.2 cells and naive L1.2 cells to increasing CXCL17 concentrations in a modified Boyden chamber. Data are shown as the mean ± SEM from four experiments.