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. 2018 Jun 6;201(2):714–724. doi: 10.4049/jimmunol.1700884

FIGURE 4.

FIGURE 4.

GPR35 is endocytosed by lodoxamide but not by CXCL17. (A) shows aAnti-HA staining of clone 23, an L1.2 line stably expressing 3xHA-GPR35 (solid histogram) with isotype control staining shown as an open histogram. (B) shows a lack of intracellular calcium flux in response to 100 nM CXCL17 in clone 23 cells. 0.1% SDS was used as a positive control to lyse cells and show successful loading with the Fura-2 dye. The data shown are from a single experiment representative of four experiments. (C) shows 3xHA-GPR35 internalization in clone 23 following incubation with different lodoxamide (LDX) concentrations at 4°C or 37°C for 15 min. (n = 3). (D) shows 3xHA-GPR35 internalization in response to 1 μM lodoxamide or 1 μM CXCL17 incubated at 4°C or 37C for 15 min (n = 4). Data are shown as the mean ± SEM for the number of experiments shown in brackets. Statistical differences between controls were confirmed by a Student t test. *p < 0.05.