FIGURE 9.

CXCL17 undergoes cleavage by mast cell chymase with little effect upon potency or efficacy in chemotaxis assays. (A) shows an alignment of the mature forms of human CXCL17, CCL15, and CCL23. The site of chymase cleavage of CCL15 and CCL23 is shown by a filled triangle. (B) shows the cleavage of CCL15 and CCL23 following an 18 hr incubation with chymase. Proteins were separated by SDS-PAGE and visualized by Coomassie Brilliant Blue staining (n = 3). (C) and (D) show the relative potency and efficacy of cleaved and uncleaved CCL15 (C) and CCL23 (D) in Boyden chamber chemotaxis assays using CCR1 transfectants (n = 3). (E) shows the cleavage of CXCL17 following an 18 h incubation with chymase. Proteins were separated by SDS-PAGE and visualized by Coomassie Blue staining (n = 3). (F) shows the relative potency and efficacy of cleaved and uncleaved CXCL17 in Boyden chamber chemotaxis assays using PGE₂-treated THP-1 cells (n = 4).