Figure 2.

miR-21 regulates IRI by modulating HIF-1α and HIF-2α expression. (A) Injection of LNA-modified anti-miR-21 (10 mg/kg) through the tail vein 1 h before kidney IR surgery or sham operation. Blocking miR-21 attenuated IR-induced up-regulation of HIF-1α and HIF-2α in the kidney. (B) Group data from A. (C) L-Mimosine (50 mg/kg) or CoCl₂ (20 mg/kg) was injected intraperitoneally 1 h before kidney IR surgery. PAS staining was performed to access the injury of the kidney. IR-induced kidney damage is mainly presented as epithelial denudation and dilation. The injury is lower in the L-Mimosine or CoCl₂-treated animals. (D) group data from C. (E) Level of creatinine in the serum. *P < 0.05, **P < 0.01, *** < 0.001, n = 6.