Skip to main content
. 2018 Jun 26;9:790. doi: 10.3389/fphys.2018.00790

Figure 4.

Figure 4

Anti-miR21 attenuated HR induced up-regulation of HIF-1α and HIF-2α and aggravated HR induced apoptosis of HK-2. Expression of miR-21, HIF-1α and HIF-2α in the HK-2 cells under hypoxia (6 h)/ reoxygenation (30 min or 1 h) condition were detected. (A) expression level of miR-21 in the HK-2 cells was increased from 30 min after reoxygenation compared with normoxia condition. (B) Protein level of HIF-1α and HIF-2α were also elevated in the HR-treated HK-2 cells. (C,D) group data from F, n = 6, *p < 0.05, **p < 0.01, ***p < 0.01 compared with normoxia condition. (E) Transfection of 50 nM LNA-modified anti-miR-21 to HK-2 cells significantly inhibited expression of miR-21 even under normoxia or HR condition. (F) Inhibition of miR-21 attenuated HR induced up-regulation of HIF-1α and HIF-2α. (G) Group data from F. *p < 0.05, **p < 0.01, ***p < 0.01, n = 6. (H) Apoptosis of the HK-2 cells was accessed by annexin-V-FITC/PI staining and determined by flow cytometry. Knockdown of miR-21 attenuated HR induced apoptosis of HK-2 cells. (I) Group data from (H). *** < 0.001, n = 3.