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. 2018 Jun 26;9:790. doi: 10.3389/fphys.2018.00790

Figure 6.

Figure 6

HR inducible miR-21 mediated HIF-1α and HIF-2α expression through PTEN/AKT/mTOR pathway. (A) HK-2 cells were transfected with LNA-modified anti-miR-21 or the scrambled control under hypoxia/reoxygenation and normoxic conditions. Western blotting was performed to analyze the expression of PTEN and phosphorylation of AKT and mTOR. HR treatment inhibited expression of PTEN, otherwise increased phosphorylation of both AKT and Mtor. (B) Group data from (A). (C) Phosphorylation of AKT was blocked by treating HK-2 cells with 50 μM LY294002 during HR. Inhibition of AKT prevents phosphorylation of mTOR and inductions of HIF-1α and HIF-2α under HR condition. (D) Group data from (C). (E) Phosphorylation of mTOR was blocked by treating HK-2 cells with 10 nM rapamycin during HR. Inhibition of mTOR reduced HIF-1α and HIF-2α stabilization. (F) Group data from (E). *p < 0.05, **p < 0.01, ***p < 0.01, n = 6.