Table 1.
Reagents and materials.
| Reagents and materials | Manufacturers | Cat. no. |
|---|---|---|
| Cobalt dichloride (CoCl2) | Sigma-Aldrich, Inc. (St. Louis, MO, USA) | 60818 |
| L-Mimosine (from Koa haole seeds) | Sigma-Aldrich, Inc. (St. Louis, MO, USA) | M0253 |
| LY294002 | Sigma-Aldrich, Inc. (St. Louis, MO, USA) | L9908 |
| Rapamycin | Gene Operation (Shanghai, China) | IPA1021 |
| Locked nucleic acid (LNA)-modified anti-scrambled oligonucleotides | Exiqon (MA, USA) | synthesized |
| Locked nucleic acid (LNA)-modified anti-miR-21 oligonucleotides | Exiqon (MA, USA) | synthesized |
| Dulbecco's modified Eagle's medium/nutrient mixture F-12 (DMEM/F12) | KeyGEN BioTECH (Nanjing, China) | KGM12500 |
| AnnexinV-FITC/PI staining kit | KeyGEN BioTECH (Nanjing, China) | KGA105 |
| Fetal bovine serum (FBS) | GIBCO (MA, USA) | 0100147 |
| AKT antibodies | Cell Signaling Technology (Danvers, MA) | 4691s |
| phospho-AKT (Ser 473) antibodies | Cell Signaling Technology (Danvers, MA) | 4060s |
| HIF1α antibodies | Cell Signaling Technology (Danvers, MA) | 14179 |
| HIF2α antibodies | Abcam (MA, UAS) | ab179825 |
| mTOR antibodies | Abcam (MA, UAS) | ab137133 |
| p-mTOR antibodies | Abcam (MA, UAS) | ab134903 |
| Anti-S6K1 antibody | Abcam (MA, UAS) | ab186753 |
| Phospho-p70 S6 Kinase (Thr389) | Cell Signaling Technology (Danvers, MA) | 9234 |
| PTEN antibodies | Abcam (MA, UAS) | ab170941 |
| NF-κB p65 (Ser536) antibodies | Abcam (MA, UAS) | ab28856 |
| NF-κB p65 antibodies | Abcam (MA, UAS) | ab16502 |
| CCR-7 antibodies | Abcam (MA, UAS) | ab32527 |
| U6 TaqMan™ MicroRNA Assays | Thermo Fisher Scientific (MA, USA) | 001973 |
| miRNA-21 TaqMan™ MicroRNA Assays | Thermo Fisher Scientific (MA, USA) | 000397 |
| Lipofectamine 2000 | Thermo Fisher Scientific (MA, USA) | 11668030 |
| Opti-MEM serum-free media | Thermo Fisher Scientific (MA, USA) | 31985 |
| Trizol | Thermo Fisher Scientific (MA, USA) | 15596-026 |
| Human HIF-1α siRNAs | Santa Cruz Biotechnology, Inc. (TX, USA) | sc-44225 |
| Human HIF-2α (EPAS-1) siRNAs | Santa Cruz Biotechnology, Inc. (TX, USA) | sc-35316 |
| Human scramble siRNAs | Santa Cruz Biotechnology, Inc. (TX, USA) | sc-37007 |
| Antibody against GAPDH | Santa Cruz Biotechnology, Inc. (TX, USA) | sc-137179 |
| Human HIF1α primers (Forward: 5'TGCAACATGGAAGGTATTGC3'; Reverse: 5'TTCACAAATCAGCACCAAGC3') | Sangon Biological Engineering Co. Ltd. (Shanghai, China) | synthesized |
| Human HIF2α primers (Forward: 5' TGGTAGCCCTCTCCAACAAG'; Reverse: 5'TCATCCGTTTCCACATCAAA') | Sangon Biological Engineering Co. Ltd. (Shanghai, China) | synthesized |
| Human GAPDH primers | Sangon Biological Engineering Co. Ltd. (Shanghai, China) | B661104 |
| PrimeScript reverse transcription reagent Kit | TaKaRa Biotechnology Co., Ltd. (Kyoto, Japan) | RR037A |
| TaqMan®MicroRNA reverse transcription reagent Kit | Thermo Fisher Scientific (MA, USA) | 4366596 |
| SYBR® Premix Ex Taq™ | TaKaRa Biotechnology Co., Ltd. (Kyoto, Japan) | RR071A |
| Creatinine Assay Kit | Bioassay system (CA, UAS) | DICT-500 |
| PE-cy5-labeled CD45 antibodies | eBioscience (CA, USA) | 35-0415-80 |
| PE-labeled CD11c antibodies | eBioscience (CA, USA) | 12-0114-82 |
| FITC-labeled MHC-2 antibodies | eBioscience (CA, USA) | 11-5321-81 |
| APC-labeled CD80 antibodies | eBioscience (CA, USA) | 17-0801-81 |
| Mouse IL-12 p70 Quantikine ELISA Kit | R&D Systems, Inc. (MN, USA) | M1270 |
| Mouse IL-6 Quantikine ELISA KIT | R&D Systems, Inc. (MN, USA) | M6000B |
| Mouse TNF-α ELISA KIT | R&D Systems, Inc. (MN, USA) | MTA00B |
| Cleaved-Caspase 3 antibody | Cell Signaling Technology (Danvers, MA) | 9579 |