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. 2018 Jun 26;9:1358. doi: 10.3389/fmicb.2018.01358

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Copyright © 2018 Guo, Zhang, Lu, Liu, Chen, Li, Deng and Wen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of the relative affinities of SAV4189 for different target sequences. (A) EMSA of His₆-SAV4189 with labeled probe A and unlabeled probes (A, B1, B2). (B) EMSA of His₆-SAV4189 with labeled probe B1 and unlabeled probes. (C) EMSA of His₆-SAV4189 with labeled probe B2 and unlabeled probes. 0.15 nM labeled probe and ∼50- and 150-fold amounts of unlabeled specific competitor probe were used in competition assays. SAV4189 concentration: 125 nM. Arrows: free labeled probes. Brackets: SAV4189-DNA complexes.