Table 1.
Strains and plasmids used in this study.
| Strain or plasmid | Description | Source |
|---|---|---|
| S. avermitilis | ||
| ATCC31267 | Wild-type (WT) strain | Laboratory stock |
| A-144 | Industrial strain | Qilu Pharmaceutical |
| D4189 | sav_4189 deletion mutant | This study |
| C4189 | sav_4189 complemented strain | This study |
| O4189 | sav_4189 overexpression strain | This study |
| O4189/A-144 | sav_4189 overexpression strain based on A-144 | This study |
| D4190 | sav_4190 deletion mutant | This study |
| D4190/A-144 | sav_4190 deletion mutant based on A-144 | This study |
| S. coelicolor | ||
| M145 | WT strain | Laboratory stock |
| O4189/M145 | sav_4189 overexpression strain based on M145 | This study |
| E. coli | ||
| JM109 | Routine cloning host | Laboratory stock |
| ET12567 | Methylation-deficient strain | MacNeil and Klapko, 1987 |
| BL21(DE) | Host for His6-SAV4189 overexpression | Novagen |
| Plasmids | ||
| pKC1139 | Multiple-copy, temperature-sensitive E. coli–Streptomyces shuttle plasmid | Bierman et al., 1992 |
| pSET152 | Integrative E. coli–Streptomyces shuttle plasmid | Bierman et al., 1992 |
| pET-28a (+) | Plasmid for His6-tagged protein overexpression in E. coli | Novagen |
| pMD18-T | TA cloning vector | TaKaRa |
| pJL117 | Plasmid carrying Streptomyces strong constitutive promoter ermE∗p | Li et al., 2010 |
| pD4189 | pKC1139 derivative for sav_4189 deletion | This study |
| pKC1139-4189 | pKC1139 derivative for sav_4189 overexpression | This study |
| pSET152-4189 | pSET152 derivative for sav_4189 complementation | This study |
| pD4190 | pKC1139 derivative for sav_4190 deletion | This study |
| pET28-4189 | pET-28a (+) derivative for His6-SAV4189 overexpression | This study |