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. 2018 Jun 29;9:687. doi: 10.3389/fphar.2018.00687

FIGURE 5.

FIGURE 5

Insulin-like growth factor (IGF) signaling regulates Nanog expression and LSC growth. (A) IGF2 significantly increased the colony formation efficiencies of KG-1a LSCs and MOLM13 LSCs. LSCs were cultured in IMDM containing bFGF (20 ng/mL), EGF (20 ng/mL), and B27. All data represent three independent experiments for each condition (∗P < 0.05). Scale bar, 100 μm. (B) Treatment with IGF2 promoted LSC proliferation. LSCs were cultured in IMDM containing bFGF (20 ng/mL), EGF (20 ng/mL), and B27 with or without IGF2 (100 ng/mL) for 72 h. The cells were then harvested and incubated with an antibody against Ki-67. Scale bar, 60 μm. (C,D) Western blot analysis showed that IGF2 increased p-IGF1R, p-Akt, and Nanog expression levels in a time-dependent manner. GAPDH was used as a loading control. The experiments were independently repeated three times.