Figure 2.

Determination of Persistence Frequency. (A) Persister frequency represented as percent survival of 1 × 10⁸ CFU/ml of B. pseudomallei exposed to 100x MIC of each compound. DMSO vehicle controls (15, 3, or 6.25%) and associated experimental conditions were tested in (B). (C) To determine the effect of DMSO, persister assays were performed with 5x MIC of the compound and 0, 5, or 10% DMSO. Groups not investigated are labeled ND. All assays were grown at 37°C for 24 h and data presented as percent survival. (D) Persister assays were repeated at 5x MIC and 10x MIC to assess dose dependency on percent survival. Error bars indicate standard deviation and asterisks indicate a statistically significant difference between treatments (P < 0.05, ^*P < 0.001, ^**P < 0.001, ^***P < 0.0001,^****; ns, no statistically difference).