Table 1.
Calculation of glycolytic and oxidative parameters.
| Glycolytic parameter | Calculation |
|---|---|
| Glycolysis (G) | (Maximal rate measurement after glucose injection through measurement prior to oligomycin (OLIGO) injection) minus (measurement prior to glucose injection) |
| Glycolytic capacity (GC) | (Maximal rate measurement after OLIGO injection through measurement prior to 2-DG injection) minus (measurement prior to glucose injection) |
| Glycolytic reserve | (GC) minus (glycolysis) |
| Non-glycolytic acidification (NGA) | Measurement prior to glucose injection |
| Oxidative parameter | Calculation |
| Basal respiration (BR) | (Last measurement prior to OLIGO injection) minus (non-mitochondrial-derived OCR value) |
| Proton leak (PL) | (Minimum rate measurement after OLIGO injection) minus (non-mitochondrial-derived OCR value) |
| ATP-linked production (ATP LINK) | (Basal rate) minus (PL) |
| Maximum respiration capacity (MAX CAP) | (Maximal rate measurement after FCCP injection) minus (non-mitochondrial-derived OCR value) |
| Non-mitochondrial-derived OCR (NON-MITO) | Minimum rate measurement after addition of rotenone/antimycin A through to the end of the assay |
| Spare respiratory capacity (SPA CAP) | (Maximum respiration capacity) minus (BR) |
This table is adapted from data provided by Agilent Technologies (18).