Fig 3.

Cisplatin is an inducer of NF-κB in muscle cells. (A) CD2F1 mice were treated with cisplatin alone as described above in legend for Figure 1. At day 17 post-injection, muscle sections were prepared and immunostained for activated p65 (phospho-p65). (B) C2C12 myotube cultures were either left alone (control) or treated with cisplatin for indicated times. Nuclear extracts were prepared and EMSA was performed. Intensity of binding complexes were quantitated by phosphoimaging and recorded underneath each lane. Fold designates the amount of binding complex over that of untreated myotubes, which was set to a value of 1.0. Separate Supershift EMSA were preformed with p50 and p65 antibodies. SS refers to supershift complexes.