Table 4.

Prevalence of IgG antibodies reacting to SV40 Tag mimotopes in serum samples from OS patients, compared to HS and BC.

Serum	Number of Patients/Individuals	Mean Age (Range) Years	Male %	Number of SV40 Tag-positive samples (%)
				Tag A	Tag D	Tag A+D
OS	249	22 (6–76)	56	94 (38)	99 (40)	87 (35)
HS	247	21 (4–76)	47	63 (26)	63 (26)	56 (23)*
BC	78	42 (25–69)	0	15 (19)	17 (22)	15 (19)°

OS, osteosarcoma affected patients; HS, healthy subjects; BC, breast cancer affected patients; IgG, immunoglobulin G; SV40, Simian virus 40; Tag, large T antigen; Tag A and Tag D, synthetic peptides/mimotopes employed in indirect ELISA to detect SV40 Tag antibodies.

The different prevalence of SV40 antibodies in OS sera compared to HS was statistically significant (^*P < 0.01).

The prevalence of SV40 antibodies in OS sera differs statistically from BC (°P < 0.01).

P values were determined using the Chi-square with Yates' correction.