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. 2018 May 24;16(2):1801–1808. doi: 10.3892/ol.2018.8797

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Figure 3. — Effects of lncRNA GAS5 on cell cycle. (A) Flow cytometry analysis of the percentage of cells in G₀/G₁, S and G₂/M phases in pc-GAS5 and pc-NC groups. (B and C) Western blot assay showed the expression levels of p-ATM, p-CHK2, p-CDC25C and p-CDK1 in pc-GAS5 and pc-NC groups. (D and E) Western blot assay showed the expression levels of p-ATM and p-CHK2 in esophageal squamous cell carcinoma tissues and their adjacent normal tissues. (F and G) Pearson's analysis of the correlation between the p-ATM expression and GAS5 expression. *P<0.05. lncRNA GAS5, long non-coding RNA growth arrest-specific transcript 5; p-ATM, phosphorylated ATM serine/threonine kinase; CHK2, checkpoint kinase 2; CDC25C, cell division cycle 25C; CDK1, cycling-dependent kinase 1; NC, negative control.