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. Author manuscript; available in PMC: 2018 Jul 9.
Published in final edited form as: J Proteome Res. 2017 Aug 7;16(9):3363–3369. doi: 10.1021/acs.jproteome.7b00381

Figure 3.

Figure 3

UbiChEM-MS analysis of Ub chains enriched from untreated asynchronous cells and nocodazole treated cells. (A) Representative deconvoluted MS spectra for all the conditions tested highlighting the three minimal digestion products: Ub1–74 (blue), GGUb1–74 (green) and 2×GGUb1–74 (orange). (B) The distribution was calculated by averaging relative abundance of each Ub1–74 species to total abundance of all three Ub1–74 species from three biological replicates for each condition (Table S2). Error bars represent standard error of the mean (SEM) for each data set. The reported calculated (calc.) and experimental (expt.) values correspond to the monoisotopic molecular weight.