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. Author manuscript; available in PMC: 2018 Jul 9.
Published in final edited form as: J Proteome Res. 2017 Aug 7;16(9):3363–3369. doi: 10.1021/acs.jproteome.7b00381

Figure 4.

Figure 4

ETD analysis of 2×GGUb1–74 species isolated from synchronized cells. ETD fragments used to deduce the site for the two diGly modifications to be at Lys11 and Lys48. The position of modified lysine is highlighted in green (top). Observed ETD fragments (c and z ions) mapped onto the sequence of Ub containing GG modifications at Lys11 and Lys48 (bottom).