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Detection of effect of ICA on the expression levels of related genes in the Wnt/β-catenin signaling pathway via semi-quantitative PCR. (A) Agarose gel electrophoresis. (B-D) Statistical charts of the relative expression levels of β-catenin, c-Myc and cyclin D1. mRNA expression levels in β-catenin, c-Myc and cyclin D1 in osteosarcoma cells were significantly decreased after ICA treatment (**p<0.01). ICA, icariin.
