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FBXO39 knockdown using shRNA in U-2OS cells. (A) Transfection efficiency was examined by fluorescence imaging. (B) FBXO39 mRNA expression was determined by reverse transcription-quantitative polymerase chain reaction. **P<0.01. (C) FBXO39 protein expression level was detected by western blot analysis in U-2OS cells. FBXO39, F-box protein 39; shRNA, short hairpin RNA; shFBXO39, cells transfected with FBXO39-targeting shRNA; shCtrl, cells transfected with control shRNA.
