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. 2018 Jun 5;16(2):2221–2228. doi: 10.3892/ol.2018.8906

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Overexpression of SOX7 inhibits the proliferation of TSCC cells in vitro. (A) The alteration of SOX7 expression in protein level was identified using western blotting in Cal27 and Tca-8113 cells after transfected with SOX7 vector or NC. (B) Cal27 and (C) Tca-8113 cell viability was detected using MTT assay after overexpression of SOX7. Relative PCNA expression was measured by RT-qPCR in (D) Cal27 and (E) Tca-8113 cells after overexpression of SOX7. Relative clone numbers were measured in (F) Cal27 and (G) Tca-8113 cells by clone formation assay after overexpression of SOX7. SOX7, sex-determining region Y-box 7; TSCC, tongue squamous cell carcinoma; NC, negative control; PCNA, proliferating cell nuclear antigen.*P<0.05, **P<0.01.