Table I.
PCR primer sequences.
| Gene | Sequence (5′-3′) |
|---|---|
| HuR RT-qPCR forward | ATTGTATGTGGTCTCCGCTGTTTG |
| HuR RT-qPCR reverse | TTTCTTTTGGGTTGAGCCTTTTTT |
| HMGA1 RT-qPCR forward | CCCGCCCACCCACGCATACACACA |
| HMGA1 RT-qPCR reverse | GCCCCCAAACCAAAAGCCCAGAGA |
| Ki67 RT-qPCR forward | GTGCTCAACAACTTCATTTCCAAC |
| Ki67 RT-qPCR reverse | AACACATTTCCTCCAAAACTCTCT |
| E-cadherin RT-qPCR forward | ATGGCTTCCCTCTTTCATCTCCTG |
| E-cadherin RT-qPCR reverse | TTCATAGTTCCGCTCTGTCTTTGG |
| Vimentin RT-qPCR forward | TCAGAATATGAAGGAGGAAATGGC |
| Vimentin RT-qPCR reverse | TCAGGGAGGAAAAGTTTGGAAGAG |
| GAPDH RT-qPCR forward | CGGAGTCAACGGATTTGGTCGTAT |
| GAPDH RT-qPCR reverse | AGCCTTCTCCATGGTGGTGAAGAC |
| Luc-HMGA1-wt forward | AAGAAAAACCTTCCCGGTGCAATCG |
| Luc-HMGA1-wt reverse | CAAGTAACTGCAAATAGGAAACCAG |
| Lenti-HuR forward | ATGTCTAATGGTTATGAAGACCACA |
| Lenti-HuR reverse | TTATTTGTGGGACTTGTTGGTTTTG |
| Lenti-HMGA1 forward | ATGAGTGAGTCGAGCTCGAAGTCCA |
| Lenti-HMGA1 reverse | TCACTGCTCCTCCTCCGAGGACTCC |
HuR, human antigen R; HMGA1, High Mobility Group AT-Hook 1; Lenti-HuR, HuR sequences inserted into pLVX-IRES-ZsGreen1; Lenti-HMGA1, HMGA1 sequences inserted into pLVX-IRES-ZsGreen1; E-cadherin, epithelial-Cadherin; Luc-HMGA1-wt, wild type HMGA1 3′UTR sequences inserted into the PMIR-Reporter plasmid; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.