Figure 2.

Functional assay for miR inhibitor treatment. (A) Clonogenic assays for SC-M1 cells were treated with miR-23a, miR-27a and miR-24 inhibitor. Colony numbers were counted using Image J. *P<0.05 vs. scramble RNA pre-treated SC-M1 cells. (B) SC-M1 cells were treated with miR-23a, miR-27a and miR-24 inhibitors mixture for 24 h and stained with DAPI. Red arrows indicate apoptotic bodies. (C) miR-23a, miR-27a and miR-24 inhibitor mixture pre-treated SC-M1 cells were implanted subcutaneously into nude mice, resulting in the observed tumors. P<0.05 vs. scramble RNA pre-treated SC-M1 cells. miR, microRNA.