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. 2018 May 25;16(2):1722–1726. doi: 10.3892/ol.2018.8815

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Copyright: © Nie et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Analysis of specificity of miR-222 stem-loop RT-PCR. Lane A, 5 µg of total RNA; lane B, RNA (<200 nt) isolated from 5 µg of total RNA; lane C, miRNA precursor (60–100 nt) isolated from 5 µg of total RNA by 15% PAGE; lane D, mature miRNA (18–24 nt) isolated from 5 µg of total RNA by 15% PAGE. PCR with 30 cycles provided a faint band of miRNA precursor, while PCR with 22 cycles failed to show this band.