Figure 4.

miR-205 directly targeted VEGFA by binding to its 3′UTR in hepatocellular carcinoma. (A) The predicted miR-205 binding site within VEGFA 3′UTR and its mutated version by site mutagenesis are presented. (B) Dual-luciferase reporter assays were performed to confirm the direct regulation of miR-205 in the 3′UTR of VEGFA. miR-205 decreased VEGFA expression at the (C) mRNA and (D) protein levels in HepG2 and HuH-7 cells. *P<0.05 vs. NC. miR, microRNA; VEGFA, vascular endothelial growth factor A; 3′UTR, 3′untranslated region; NC, negative control; Wt, wild-type; Mut, mutant.