Treatment of β-elemene-paclitaxel inhibits growth and arrests cell cycle of U-2OS cells. (A) Combined treatment of β-elemene (100 mg/ml) and paclitaxel (20 mg/ml) significantly inhibits growth of U-2OS cells. (B) Combined treatment of β-elemene (100 mg/ml) and paclitaxel (20 mg/ml) suppresses growth of U-2OS cells in time-dependent manner. (C-E) Treatment of (C) β-elemene (D) paclitaxel and β-elemene-paclitaxel (E) arrested U-2OS cells cycle at G1 and S, G1, S phase respectively. (F) Combined treatment of β-elemene-paclitaxel decreases gene CDK1, cyclin-B1 and P27 expression levels in U-2OS cells, **P<0.01. CDK1, cyclin-dependent kinase 11.