Table I.
Gene name | Sequence |
---|---|
GPR124 | Reverse: 5′-GGTCGTTCTACTGGGCTGATT-3′ |
Forward: 5′-AGCAAGAGGGGATTTCACAAT-3′ | |
TIMP-1 | Forward: 5′-GTCAGTGAGAAGCAAGTCGA-3′ |
Reverse: 5′-ATGTTCTTCTCTGTGACCCA-3′ | |
TIMP-2 | Forward: 5′-TGGGGACACCAGAAGTCAAC-3′ |
Reverse: 5′-TTTTCAGAGCCTTGGAGGAG-3′ | |
MMP-3 | Reverse: 5′-CTTCTTCAAGGACCGGTCA-3′ |
Forward: 5′-GCTGGCTGAGTACCAGTA-3′ | |
MMP-9 | Reverse: 5′-TGGGCTACGTGACCTATGAC-3′ |
Forward: 5′-GCCCAGCCCACCTCCACTCC-3′ | |
VEGF | Reverse: 5′-GCACCCATGGCAGAAGGAGGAG-3′ |
Forward: 5′-GTGCTGACGCTAACTGACC-3′ | |
Endostatin | Forward: 5′-ATGCACAGCCACCGCGACTT-3′ |
Reverse: 5′-CTTCATGACTGCCTCCAAGTAG-3′ | |
Bcl-2 | Forward: 5′-CTCAGCCAGCCAGTGACATA-3′ |
Reverse: 5′-CCGTGCTCCAGATACAT-3′ | |
Bcl-w | Forward: 5′-AGAGTGGACCACACTGCGC-3′ |
Reverse: 5′-ACATCCCAACGGTCATCGTA-3′ | |
Bax | Reverse: 5′-GGAGGGGATCAGTATATACA-3′ |
Forward: 5′-GAAGATGGAGAGATGG-3′ | |
Caspase-3 | Reverse: 5′-CTGTGAGATCACTGGCTTTG-3′ |
Forward: 5′-TTGGAGGGAACAGACGAG-3′ | |
CDK1 | Reverse: 5′-ACAGTGCATCATCGCTGTTC-3′ |
Forward: 5′-CCGGAGAGGAGACTTCACAG-3′ | |
Cyclin-B1 | Reverse: 5′-GAAAGCATCCAGCAATAGGC-3′ |
Forward: 5′-TAAGGAAGCCTGGAGCACAG-3′ | |
P27 | Reverse: 5′-CATGGTGAAACCCCGTCTCTA-3′ |
Forward: 5′-GCCTCAGCCTCCCGAGTAG-3′ | |
MDR1 | Reverse: 5′-GGGCAGAATCTTTCCACCA-3′ |
Forward: 5′-TTAAATGTATACCCAAAGACAA-3′ | |
LRP | Reverse: 5′-CGAATGGGTGTTTTCACATATG-3′ |
Forward: 5′-CTTCAATTGTATTCAGGATGG-3′ | |
TS | Reverse: 5′-CACCCTCAATATTTGGAA-3′ |
Forward: 5′-CCGTTGTTGTAGGACTAATGAA-3′ | |
β-actin | Reverse: 5′-CGGAGTCAACGGATTTGGTC-3′ |
Forward: 5′-AGCCTTCTCCATGGTCGTGA-3′ |
GPR124, G-protein coupled receptor 124; TIMP, TIMP metallopeptidase inhibitor; MMP, matrix metallopeptidase; VEGF, vascular endothelial growth factor; Bcl, B-cell lymphoma; Bax, Bcl-2 associated X, apoptosis regulator; CDK1, cyclin dependent kinase 1; MDR1, ATP binding cassette subfamily B member 1; LRP, LDL receptor related protein.