Figure 4.

Expression of REV-ERBα is decreased in human gastric cells. (A) Expression of REV-ERBα in the normal gastric epithelial GES-1 cell line and human gastric cancer SGC-7901 and BGC-823 cell lines was measured by western blot analysis. β-actin was used as a loading control. (B) Relative protein expression of REV-ERBα was normalized to that of β-actin. (C) The mRNA levels of REV-ERBα was measured by reverse transcription quantitative polymerase chain reaction in the normal gastric epithelial GES-1 cell line and human gastric cancer SGC-7901 and BGC-823 cell lines. Data are presented as the mean ± standard deviation. n=3. *P<0.05 vs. normal gastric epithelial GES-1 cell line. REV-ERBα, nuclear receptor subfamily 1 group D member 1.