Figure 3.

CCAT1 regulates the expression levels of epithelial-to-mesenchymal transition-associated markers. (A) N-cadherin, vimentin and E-cadherin protein expression levels in lung adenocarcinoma cells were analyzed by western blotting. CCAT1 overexpression promoted the expression of N-cadherin and vimentin, while suppressing E-cadherin expression in vitro. β-actin was used as a loading control. (B) Average values of integrated optical density of the bands were calculated by analyzing the results of five repeated experiments.. Data are presented as the mean ± standard error of the mean. *P<0.01. Corresponding P-values determined by Mann-Whitney U-test are indicated. CCAT1, colon cancer-associated transcript 1; siCCAT1, small interfering RNA against colon cancer-associated transcript 1; E-cad, E-cadherin, N-cad, N-cadherin; vim, vimentin.