Figure 2.

CYP27A1 promotes proliferation in glioblastoma LN-229 cells. (A) Western blot analysis for CYP27A1 in LN-229 cells. LN-229 cells were transfected with CYP27A1-expressing plasmids or the empty vector (mock). β-actin was used as the loading control. n=3. (B) MTT assay for LN-229 cells. LN-229 cells were transfected with CYP27A1-expressing plasmids or the empty vector (mock) and cellular viability was then measured at the indicated time-points using an MTT assay. n=3. Error bars indicate standard error of the mean.