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. 2018 May 30;16(2):1439–1448. doi: 10.3892/ol.2018.8846

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Copyright: © Xin et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 9. — miR-204 is downregulated in glioblastoma and its overexpression inhibits proliferation, migration and invasion in glioblastoma U87MG cells. (A) Northern blot analysis for miR-204 in glioblastoma tissues (C) and adjacent normal tissues (N). β-actin was used as the loading control. n=7. (B) MTT assay for U87MG cells. U87MG cells were transfected with pre-miR-204 (precursors miRNA) and control miR (mock) and cell viability was then measured at the indicated time points using an MTT assay. n=3. (C) BrdU incorporation assay for U87MG cells transfected with pre-miR-204 or control miR (mock). n=3. (D) Matrigel invasion assay and Transwell migration assay for U87MG cells transfected with pre-miR-204 or control miR (mock). n=3. Error bars indicate standard error of the mean. miR, microRNA; BrdU, bromodeoxyuridine.