Table I.
Primer name | Sequence |
---|---|
Cloning primers | |
GP5+ | 5′-TTTGTTACTGTGGTAGATACTAC-3′ |
GP6+ | 5′-GAAAAATAAACTGTAAATCATATTC-3′ |
MY09 | 5′-CGTCCMARRGGAWACTGATC-3′ |
MY11 | 5′-GCMCAGGGWCATAAYAATGG-3′ |
E2-forward | 5′-AACGAAGTATCCTCTCCTGAAATTATTAG-3′ |
E2-reverse | 5′-CCAAGGCGACGGCTTTG-3′ |
E6-forward | 5′-GAGAACTGCAATGTTTCAGGACC-3′ |
E6-reverse | 5′-TGTATAGTTGTTTGCAGCTCTGTGC-3′ |
E7-forward | 5′-CCGGACAGAGCCCATTACAA-3′ |
E7-reverse | 5′-CGAATGTCTACGTGTGTGCTTTG-3′ |
PC04 | 5′-CAACTTCATCCACGTTCACC-3′ |
GH20 | 5′-GAAGAGCCAAGGACAGGTAC-3′ |
qPCR primers and Taqman probe | |
E2-forward | 5′-AACGAAGTATCCTCTCCTGAAATTATTAG-3′ |
E2-reverse | 5′-CCAAGGCGACGGCTTTG-3′ |
E2-probe | 5′-FAM-CACCCCGCCGCGACCCATATAMRA-3′ |
E6-forward | 5′-GAGAACTGCAATGTTTCAGGACC-3′ |
E6-reverse | 5′-TGTATAGTTGTTTGCAGCTCTGTGC-3′ |
E6-probe | 5′-FAM-CAGGAGCGACCCAGAAAGTTACCACAGTTTAMRA-3′ |
β-globin-F | 5′-TGGGTTTCTGATAGGCACTGACT-3′ |
β-globin-R | 5′-AACAGCATCAGGAGTGGACAGAT-3′ |
β-globin-probe | 5′-FAM-TCTACCCTTGGACCCAGAGGTTCTTTGAGTTAMRA-3′ |
qPCR, quantitative polymerase chain reaction; FAM, 6-carboxyfluorescein; TAMRA, tetramethylrhodamine.