Table II.
Sample conditions, PCR amplification, HPV infection status, ISH signal status and p16INK4a score in branchial cleft cyst.
| PCR amplification | Integration | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Patient | Sex | Age, years | Disease | Sample | GP5+/GP6+ | MY09/MY11 | E2-F/E2-R | E6-F/E6-R | E7-F/E7-R | HPV type | Viral load (copies/50 ng DNA) | E2/E6 | Status | ISH signal status | p16 score |
| 1 | Male | 2 | Branchial cleft cyst | FFPE | – | – | + | + | + | 16 | 1.65×106 | 1.03 | Episomal | D | 0 |
| 2 | Male | 29 | Branchial cleft cyst | FFPE | + | + | + | + | + | 16 | 7.39×102 | 0.56 | Mixed | D, P | 1 |
| 3 | Female | 18 | Branchial cleft cyst | FFPE | – | – | + | + | – | 16 | 2.21×104 | 0.03 | Mixed | D, P | 1 |
| 4 | Female | 6 | Branchial cleft cyst | Fresh-frozen | – | – | + | + | Not tested | 16 | 9.21×103 | 0.03 | Mixed | D, P | 1 |
| 4 | Cyst fluid | Fresh-frozen | – | – | + | + | Not tested | 16 | 7.10×102 | 0.49 | Mixed | NA | NA | ||
| 5 | Male | 16 | Branchial cleft cyst | FFPE | – | – | – | – | – | No | (−) | 1 | |||
| 6 | Female | 9 | Branchial cleft cyst | FFPE | – | – | – | – | – | No | (−) | 1 | |||
| C | Male | 50 | Metastatic lymph node from oropharyngeal cancer | Fresh-frozen | + | + | + | + | + | 16 | 1.06×106 | 0.92 | Mixed | D, P | 4 |
C, control; D, diffuse; FFPE, formalin-fixed paraffin-embedded; ISH, in situ hybridization; P, punctate; NA, not available; -, no amplification; +, amplification; (−) no positive signal.