Table I.
Primers for reverse transcription-quantitative polymerase chain reaction and promoter analysis of Per1 and Per2 genes.
| Gene | Genbank accession no. | Forward (5′-3′) | Reverse (5′-3′) | Position |
|---|---|---|---|---|
| PER1 | NM_002616.2 | GGACATGACCTCTGTGCTGA | CATCAGGGTGACCAGGATCT | 3,688–3,887 |
| PER2 | NM_022817.2 | ACAGCTTTGGCTTCTGGTGT | TATTGGCCATCATGGTCTGA | 4,574–4,774 |
| GAPDH | NM_002046.5 | GTCAGTGGTGGACCTGACCT | TGAGGAGGGGAGATTCAGTG | 908-1,307 |
| ACTB | NM_001101.3 | TCCCTGGAGAAGAGCTACGA | AGCACTGTGTTGGCGTACAG | 787–980 |
| PER1p | – | TGTCTCTCCCCTCCTCTCAAa | AGATACGCTGCGCCTCTTTAa | −437 to −241 |
| PER2p | – | AGGAACCGACGAGGTGAAC | CCGCTGTCACATAGTGGAAA | −410 to −217 |
Primers were designed with Primer3 software
a
correspond to those reported by Gery et al (35). Per, Period circadian regulator; ACTB, β-actin; PER1/2p, Per1 and 2 gene promoter.