Intensity of cytoplasmic WT1 intensity in pancreatic ductal adenocarcinoma patients following surgical resection. (A) Negative control samples were stained with IgG monoclonal antibody. Cells positive for cytoplasmic WT1, as determined using an anti-human WT1 monoclonal antibody (6F-H2), were graded as (B) weak, (C) moderate or (D) strong (magnification, ×200). WT1, Wilms' tumor 1; IgG, immunoglobulin G.