Figure 1.

Characterization of A549-GFT and A549 cells. (A) Cells were maintained in 96-well plates and cell proliferation at indicated time intervals was assessed by MTT assay. Growth curves of the two cells were drawn. (B) Cells were incubated in 100 µl medium for 24 h, and then were treated with 0, 1, 3, 5, 7, 9 and 18 µmol/l gefitinib for 48 h. Cell proliferation was evaluated by MTT assay. The IC₅₀ values of the two cell lines were calculated. (C) A549-GFT and A549 cells were exposed to 2 µmol/l gefitinib for 48 h, and then cell morphology was visualized by microscopy. (D) E-cadherin and Vimentin expression in A549-GFT and A549 cells were assessed by western blot analysis. GAPDH was detected as an internal standard. *P<0.05 vs. A549 cells at 48 h.