Figure 4.

MAP3K10 is targeted by miR-155-5p in AGS-1 cells. (A) The intact and mutated versions of the MAP3K10 3′UTR and miR-155-5p are presented. (B) miR-155-5p mimics inhibited the activity of luciferase in the plasmid containing the 3′UTR of the WT MAP3K10 gene. (C) AGS-1 cells were transfected with miR-155-5p mimics, and 48 h later, the MAP3K10 protein expression level was analyzed by western blotting. Data are presented as the mean±standard deviation, and the experiments were repeated 3 times. *P<0.05 miR-NC vs.miR-155-5p MAP3K10, mitogen-activated protein kinase kinase kinase 10; miR, microRNA; 3′UTR, 3′untranslated region; hsa, homo sapiens; NC, negative control; WT, wild type.