Figure 5.

Synergistic anti-angiogenesis by aspirin plus erlotinib. (A) HUVEC cells were gently scratched with a sterile pipette tip and then washed with PBS three times. Next, HUVEC cells were incubated with 2 mM aspirin, 2.5 µM erlotinib or the combination for 24 h. Photographs were taken by microscope. (B) HUVEC cells were seeded in the upper chamber and then cells were incubated with 2 mM aspirin, 2.5 µM erlotinib or the combination for 24 h. Cell invasion experiments were performed. (C) Quantification of invaded HUVEC cells was counted in Matrigel Transwell invasion assays. (D) CAMs were incubated with aspirin (5 µg) and/or erlotinib (1 µg) for 48 h. Inhibitory effect of aspirin plus erlotinib on angiogenesis was detected. (E) Blood vessel branches were counted to quantify angiogenesis.