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. 2018 Jun 5;16(2):2517–2524. doi: 10.3892/ol.2018.8899

Figure 5.

Figure 5.

AG490 inhibits cell viability and induces apoptosis of SGC-7901 gastric cancer cells. SGC-7901 cells were treated with PA (80 µM) in the absence or presence of AG490 (100 µM). (A) The activation of JAK2 and STAT3 was analyzed by western blotting. (B) CCK-8 assay was performed to examine cell viability. (C) Annexin V-FITC/PI staining was used to detect cell apoptosis. (D) Representative cell apoptosis profiles of SGC-7901 cells after PA and/or AG490 treatment. (E) The expression of Bcl-2, Bax, cyt-c and caspase-3 proteins was analyzed by western blotting. These experiments were performed on the same membrane. *P<0.05, **P<0.01 and ***P<0.001 vs. the control group. #P<0.05, ##P<0.01, ###P<0.001 vs. PA or AG490 groups. Bax, BCL2 associated X protein; Bcl-2, B-cell lymphoma 2; cyt-c, cytochrome c; FITC, fluorescein isothiocyanate; JAK2, Janus kinase 2; PA, pachymicacid; p-, phosphorylated; PI, propidium iodide; STAT3, signal transducer and activator of transcription 3.