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Immunohistochemical staining for nestin and BRAFV600E. (A) Stage IV SM showing strong expression of nestin. (B) Stage II SM showing weak expression of nestin. (C) Absence of immunoreactivity to BRAF in gastrointestinal melanoma. (D) Strong expression of BRAF in SM. Counterstaining was by hematoxylin. Magnification, −600. SM, skin melanoma.
