Figure 5.

ROD1 suppresses tumor growth in a nude mouse xenograft model. (A) Western blot analysis of β-catenin (Y333) in tumor tissues and (B) quantification of its expression levels. (C) Tumor regression observed in xenografts treated with Ad-vector, Ad-ROD1 and Ad-shROD1. (D) Representative images of tumors taken on day 15 following inoculation of MDA-MB-231 cells. The revealed no significant difference in tumor growth, suggesting that tumors were induced by continued infection of MDA-MB-231 cells. Analysis of Ki67 expression in tumor tissues, as detected by (E) immunohistochemistry and (F) quantified. ^#P<0.05 vs. Ad-Vector. Ad, adenovirus; ROD1, regulator of differentiation 1; sh, short hairpin. Scale bars, 100 µm (Fig. 5E).