Table 1.
Bacteria and plasmids used in this study.
Name/species | Genotype/characteristicsa | References |
---|---|---|
Plasmids | ||
pB10 | Wild type IncP-1 plasmid isolated from activated sludge | Schlüter et al. (2003) |
pBELX | pEX-A derivative (pUC18-based) containing qPCR target sequences for trfA of IncP-1 plasmids, the Eubacterial 16S rRNA gene | Bellanger et al. (2014b) |
pNORM1 | pEX-A derivative (pUC18-based) containing the qPCR target sequences for intI of class 1 integron, the Eubacterial 16S rRNA gene | Gat et al. (2017) |
Bacillus subtilis | ||
LMG 7135T | ATCC 6051, Type strain | BCCM/LMGb |
CM291 | RifR derivative of LMG 7135T | This work |
CM295 | CM291(pB10), RifR, AmxR, StrR, SulR, TetR | This work |
Cupriavidus metallidurans | ||
AE815 | plasmid free and RifR derivative of wild type strain CH34 | Springael et al. (1993) |
CM124 | AE815(pB10), RifR, AmxR, StrR, SulR, TetR | Bellanger et al. (2014a) |
Delftia acidovorans | ||
CM122 | Delftia acidovorans, RifR, KanR | Eva Top lab strain collection |
CM294 | CM122(pB10), RifR, KanR, AmxR, StrR, SulR, TetR | This work |
Escherichia coli | ||
DH5α | ϕ80lacZΔacZerecA1endA1gyrA96 (NalR) thi-1 hsdR17(rK-mK+) supE44 relA1 deoR Δ(lacZYAargF) U169 | Sambrook et al. (1989) |
MG1655 | Sequenced λ- and F-derivative of strain K-12 | Blattner et al. (1997) |
CM102 | DH5α(pB10), NalR, AmxR, StrR, SulR, TetR | Schlüter et al. (2003) |
CM125 | NalR derivative of MG1655 | Bellanger et al. (2014a) |
CM278 | CM125(pB10), NalR, AmxR, StrR, SulR, TetR | This work |
Pseudomonas fluorescens | ||
CIP 69.13T | ATCC 13525, Type strain | ATCCc |
CM292 | RifR derivative of wild type strain CIP 69.13T | This work |
CM297 | CM292(pB10), RifR, AmxR, StrR, SulR, TetR | This work |
Pseudomonas putida | ||
SM1443 | KT2442 (RifR) with a mini-Tn5-lacIq insertion | Christensen et al. (1998) |
CM236 | SM1443(pB10), RifR, AmxR, StrR, SulR, TetR | This work |
Shewanella oneidensis | ||
CM87 | RifR derivative of wild type strain MR-1T | Schwalb et al. (2003) |
CM293 | CM87(pB10), RifR, AmxR, StrR, SulR, TetR | This work |
aAntibiotic resistance phenotype: AmxR, amoxicillin; KanR, kanamycin; NalR, nalidixic acid; RifR, rifampicin; StrR, streptomycin; SulR, sulfamides; TetR, tetracycline. bBelgian coordinated collections of microorganisms. cAmerican Type Culture Collection.