Table 2.

Polymerase chain reaction and sequencing primers of RET gene

Exon	Sense primer	PCR product (base pairs)	T (°C)
RET-1	5′- GCACCCGCCATCCAGACC -3′	567	62
RET-1	5′- TCTCCTGCCGAAACAGAACTC -3′
RET-2	5′- CGGCTTGGATGATTGAGAATAGG-3′	583	58
RET-2	5′- GTGATAAGGGCGGCTTGAGG-3′
RET-3	5’-TCCTCCCCATTCCCGACTG − 3′	573	56
RET-3	5′- AGGCAGGCAAGATTCCAACC-3’
RET-4	5’-CAACCAGCACGAGTGAGGAC -3’	640	60
RET-4	5’-ACGGAGGCGAAGGACAAATG −3’
RET-5	5’-AACACACATCTGGTCCACCTATG − 3’	375	56
RET-5	5′- AAGAGCGAGCACCTCATTTCC-3’
RET-6	5’-GTGTGAAAGTGCGTGTTTGC -3’	428	60
RET-6	5’-CAGTCTACTCTGTGCTGGTTG −3’
RET-7	5′- GGACTTAGGCTGTGTGGGAATC-3’	516	58
RET-7	5′- -CTGGAAGGAGCAACCATTTACTG-3’
RET-8	5’-GCTGGTGCTGTTCCCTGTC -3’	375	62
RET-8	5’-CACTGCTGCCCGCTATGC -3’
RET-9	5’-CCTCCAGTTGCTCCTCCCTAG −3’	470	58
RET-9	5′- CTGCTTCTGAAATCTGTGTGTGC-3’
RET-10	5’-GGCAGAGTCCTTTGTTCAGATG −3’	452	58
RET-10	5′- CAGCAATTTCCTCCCTTGTTGG-3’
RET-11	5’-GAGCCTCTGTCTCCATCTGTAAG −3’	606	56
RET-11	5’-CTCGTCTGCCCAGCGTTG −3
RET-12	5’ GCAGAGACAGGCAGCGTTG--3’	499	58
RET-12	5′- CCTTCCAGGGAGAGCAAAGTC-3’
RET-13	5′- AGAAGCCTCAAGCAGCATCG-3’	432	58
RET-13	5’-TCAGCCCGTGGACTCAGC-3’
RET-14	5′- GGCAGAGAGCAAGTGGTTCAAG-3’	618	60
RET-14	5′- GGGCGTGGTGGAGTCAGG-3’
RET-15	5′- CCACACACCACCCCTCTG-3’	401	58
RET-15	5’-GGCTGAGCGGAGTTCTAATTG −3’
RET-16	5’-CACTCCTCTGGTTACTGAAAGC -3’	370	56
RET-16	5′- CATTTGCCTCACGAACACATC-3’
RET-17	5′- CCAGACCCAGGCTGACATC-3’	449	60
RET-17	5′- ATCTACTGCCACACCCAAGG-3’
RET-18	5′- AGGGTGCGATGGCTGTGG-3’	352	58
RET-18	5′- AGGGTTCAATCTGCTGTCTGC-3’
RET-19	5’-CTTGGAGAGGTCAGGAGATTGG −3’	453	58
RET-19	5’-AATGCCTAAATGTAAACTGGATGC -3’
RET-20	5′- TTGGAAACCTGGAACACAAAACC-3’	430	62
RET-20	5′- GCCACCTGGGAACTGAACAC-3’

PCR polymerase chain reaction, RET rearranged during transfection, T annealing temperature for the PCR reactions